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ACD/MS Workbook Suite を用いたLC-MS 分析による\n発芽玄米酒粕のアンジオテンシン変換酵素阻害活性ペプチドの同定
https://u-nagano.repo.nii.ac.jp/records/1252
https://u-nagano.repo.nii.ac.jp/records/12527ea24b97-718f-4ab9-96ea-04ee5c4e11d8
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
kentan72-04 小木曽.pdf (1.2 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper_02(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-08-01 | |||||
| タイトル | ||||||
| タイトル | ACD/MS Workbook Suite を用いたLC-MS 分析による\n発芽玄米酒粕のアンジオテンシン変換酵素阻害活性ペプチドの同定 | |||||
| タイトル | ||||||
| タイトル | Identification of the angiotensin-converting enzyme inhibitory\nactivity peptide of the germinated brown rice sake-lees by\nLC-MS analysis using ACD/MS Workbook Suite | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ACE 阻害 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 発芽玄米酒粕 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 簡易同定方法 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ACD | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
小木曽, 加奈
× 小木曽, 加奈× 古田, 一匡× 岡崎, 光雄 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Until now, we have made low-salt bread using the germinated brown rice sake-lees. We would like to utilize the germinated brown rice sake-lees for making bread, which is in future. Angiotensin-Converting Enzyme (ACE) inhibitory activities were measured in germinated brown rice sake-lees, germinated brown rice liquor, and refined sake in vitro. As a result, the ACE inhibitory activities (germinated brown rice sake-lees, germinated brown rice liquor and refined sake) were 0.15 mg/ml, 1.15 mg/ml and 1.55 mg/ml with an IC50 level, respectively. Since ACE inhibitory activity of the germinated brown rice liquor was higher than that of the refined sake, the germinated brown rice-derived ingredients was suggested to have some ACE inhibitory activity. In addition, the ACE inhibitory activity of the germinated brown rice sake-lees was higher than that of the germinated brown rice liquor. Taken together, the germinated brown rice sake-lees were expected to remain more inhibitory activity than the liquor. As a method to easily search and identify the functional ingredients of the food, we conducted an examination to develop a method for identification of the functional-dipeptide from germinated brown rice sake-lees extract. From the LC-MS analysis results, we searched dipeptide with the ACE inhibitory activity using the software ACD/MS Workbook Suite (Advanced Chemistry Development, Inc., Canada) with IntelliTarget function and MS Match function. Comparing the two functions, MS Match function showned that dipeptide Val-Pro with the hypotensive effect was present in germinated brown rice sakelees. The effective dipeptide was identified easily by using MS Match function from LC-MS results |
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| 書誌情報 |
長野県短期大学紀要 en : Journal of Nagano Prefectural College 巻 72, p. 15-22, 発行日 2018-02 |
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| 出版者 | ||||||
| 出版者 | 長野県短期大学 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0286-1178 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00179035 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
