@article{oai:u-nagano.repo.nii.ac.jp:00001252,
 author = {小木曽, 加奈 and 古田, 一匡 and 岡崎, 光雄},
 journal = {長野県短期大学紀要, Journal of Nagano Prefectural College},
 month = {Feb},
 note = {application/pdf, Until now, we have made low-salt bread using the germinated brown rice sake-lees. We would like to
utilize the germinated brown rice sake-lees for making bread, which is in future.
Angiotensin-Converting Enzyme (ACE) inhibitory activities were measured in germinated brown rice
sake-lees, germinated brown rice liquor, and refined sake in vitro.
　As a result, the ACE inhibitory activities (germinated brown rice sake-lees, germinated brown rice
liquor and refined sake) were 0.15 mg/ml, 1.15 mg/ml and 1.55 mg/ml with an IC50 level, respectively.
Since ACE inhibitory activity of the germinated brown rice liquor was higher than that of the refined
sake, the germinated brown rice-derived ingredients was suggested to have some ACE inhibitory
activity. In addition, the ACE inhibitory activity of the germinated brown rice sake-lees was higher than
that of the germinated brown rice liquor. Taken together, the germinated brown rice sake-lees were
expected to remain more inhibitory activity than the liquor.
　As a method to easily search and identify the functional ingredients of the food, we conducted an
examination to develop a method for identification of the functional-dipeptide from germinated brown
rice sake-lees extract. From the LC-MS analysis results, we searched dipeptide with the ACE inhibitory
activity using the software ACD/MS Workbook Suite (Advanced Chemistry Development, Inc., Canada)
　with IntelliTarget function and MS Match function. Comparing the two functions, MS Match function
showned that dipeptide Val-Pro with the hypotensive effect was present in germinated brown rice sakelees.
The effective dipeptide was identified easily by using MS Match function from LC-MS results},
 pages = {15--22},
 title = {ACD/MS　Workbook Suite を用いたLC-MS 分析による\n発芽玄米酒粕のアンジオテンシン変換酵素阻害活性ペプチドの同定},
 volume = {72},
 year = {2018}
}